Products Details

SCRUB TYPHUS IgM MICROLISA

INTENDED USE

 

Scrub Typhus IgM Microlisa is designed for in vitro qualitativedetection of Scrub Typhus IgM

antibodies in human serum or plasma and is used as a screening test fortesting of collected

blood samples suspected for Scrub Typhus Positive.




SALIENT FEATURES:

 

·        Qualitative Detection ofScrub Typhus IgM antibodies in Human Serum/Plasma

·        Principle; Based on“Indirect ELISA”.

·        Breakaway microwell strips.

·        Color coded reagents tomonitor procedural steps.

·        Assay procedure : 75minutes. 

·        Longer shelf life : 18month at 2-8°C

·        Pack size:  96 Test.

·        Sensitivity: 100% &Specificity: 98.58%

·        Prompt service back-up


PRINCIPLE

 

Scrub Typhus IgM Microlisa test is an enzyme immunoassay based on“Indirect ELISA”.

Recombinant protein representing immunodominant epitopes of Scrub Typhusare coated onto

microtiter wells. Specimens and controls are added to the microtiterwells and incubated.

IgM antibodies to Scrub Typhus if present in the specimen, will bind tothe specific Scrub

Typhus antigen absorbed onto the surface of the wells. The plate is thenwashed to remove

unbound material. Horseradish peroxidase (HRP) conjugated Anti-Human IgMis added to each

well. This conjugate will bind Scrub Typhus antigen-antibody complexpresent. Finally substrate

solution containing chromogen and hydrogen peroxide is added to thewells and incubated. A

blue color will develop in proportion to the amount of Scrub Typhus IgMantibodies present in

the specimen. The color reaction is stopped by a stop solution. Theenzyme substrate reaction

is read by EIA reader for absorbance at a wavelength of 450 nm. If thesample does not contain

Scrub Typhus IgM antibodies then enzyme conjugate will not bind and thesolution in the wells

will be either colorless or only a faint background color develops.


ORDERING INFORMATION

 

PRODUCT NAME

PACK SIZE

CATALOG NO.

SCRUB TYPHUS IgM MICROLISA

 

96 Test 

IR099096

 

PERFORMANCE CHARACTERISTICS (IN HOUSE EVALUATION) OF SCRUB TYPHUS IgMMICROLISA

 

No. of Samples

Status

SCRUB TYPHUS IgM MICROLISA

 

 

Positive

Equivocal

Negative

20

Scrub Typhus Positive

20

0

0

70

Scrub Typhus Negative

1

0

69

 

Sensitivity: 100% Specificity : 98.58%