Chikungunya IgM Microlisa

Intended Use

Chikungunya IgM Microlisa is designed for in vitro qualitative detection of Chikungunya IgM in human serum or plasma and is used as a screening test for testing of collected blood samples suspected for Chikungunya.

Salient Features

• Principle : “MAC Capture ELISA”.
• Microwells Elisa test for detection of Chikungunya specific IgM Antibodies in Human serum & plasma.
• Sensitivity: 99.35% & Specificity: 99.93%.
• Color coded reagents to monitor procedural steps.
• Assay procedure: 105 mins.
• Prompt service back-up.
• Longer Shelf life: 24 months at 2-8° C.
• Convenient pack size:96 Tests

Principle

Chikungunya IgM Microlisa test is an enzyme immunoassay based on “MAC Capture ELISA”.
Anti-human IgM antibodies are coated onto microtiter wells. Specimens, controls and calibrator are added to the microtiter wells and incubated.
IgM antibodies to Chikungunya if present in the specimen, will bind to the anti-human IgM antibodies absorbed onto the surface of the wells. The plate is then washed to remove unbound material. Chikungunya antigen is added to each well. This antigen will bind to human antibodies against chikungunya anti-human IgM complex present.

The plate is washed to remove unbound material. Enzyme conjugate (anti-chikungunya antibodies conjugated Horseradish peroxidase (HRPO)) is added to each well. This conjugate will bind Chikungunya antigen present. Finally substrate solution containing chromogen and hydrogen peroxide is added to the wells and incubated. A blue colour will develop in proportion to the amount of Chikungunya antibodies present in the specimen. The colour reaction is stopped by a stop solution. The enzyme substrate reaction is read by EIA reader for absorbance at a wavelength of 450 nm. If the sample does not contain Chikungunya IgM antibodies then enzyme conjugate will not bind and the solution in the wells will be either colourless or only a faint background colour develops.

Ordering Information

Performance Characteristics Of Chikungunya IgM Microlisa

Sensitivity: 99.35%
Specificity: 99.93%