T4 Quanti Microlisa

Test Kit Type: T4 Test Kit (ELISA)
Longer Shelf Life: 18 Months at 2-8°C
Linearity Range: 0.4 µg/dl to 25 µg/dl
Analytical Sensitivity: 0.4 µg/dl

Intended Use

T4 Quanti Microlisa is designed for in-vitro quantitative determination of Total Thyroxine (T4) in human serum or plasma.

Salient Features

  • Principle : Based on competitive immunoassay
  • Quantitative detection of Total Thyroxine (T4) in Human Serum / Plasma.
  • Linearity Range: 0.4 µg/dl to 25 µg/dl
  • Analytical Sensitivity 0.4 µg/dl
  • Breakaway microwell strips.
  • Color coded reagents to monitor procedural steps.
  • Assay procedure: 75 mins.
  • Prompt service back-up
  • Longer shelf life: 18 Months at 2-8°C.
  • Convenient pack size: 96 tests.s.

Principle

T4 Quanti Microlisa is an enzyme immuno assay based on competitive ELISA. Microwells are coated with anti-thyroxine antibodies. Sample is added to the microwell followed by addition of enzyme conjugate (T4 labelled with HRPO).

Binding of T4 is detected by Enzyme Conjugate. Incubation is followed by a washing step to remove unbound components. The color reaction is started by addition of substrate and stopped after a defined time. The color intensity is inversely proportional to the concentration of T4 in the sample.

Ordering Information

Product Name Pack Size Catalog No.
T4 Quanti Microlisa 96 Tests IR310096

Performance Characteristics (Internal Evaluation) Of T4 Quanti Microlisa Test

  1. Accuracy : The kit has been tested using clinical samples in comparison with commercially available reference immunoassay test kits (ELISA /CLIA/ELFA) and the Accuracy co-efficient is > 0.985
  2. Precision :
    1. The test results are repeatable in Within run precision testing with CV <10%.
    2. The test results are reproducible in between run precision testing with CV <15%.

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