T4 Quanti Microlisa

Intended Use

T4 Quanti Microlisa is designed for in-vitro quantitative determination of Total Thyroxine (T4) in human serum or plasma.

Salient Features

• Principle : Based on competitive immunoassay
• Quantitative detection of Total Thyroxine (T4) in Human Serum / Plasma.
• Linearity Range: 0.4 µg/dl to 25 µg/dl
• Analytical Sensitivity 0.4 µg/dl
• Breakaway microwell strips.
• Color coded reagents to monitor procedural steps.
• Assay procedure: 75 mins.
• Prompt service back-up
• Longer shelf life: 18 Months at 2-8°C.
• Convenient pack size: 96 tests.s.

Principle

T4 Quanti Microlisa is an enzyme immuno assay based on competitive ELISA. Microwells are coated with anti-thyroxine antibodies. Sample is added to the microwell followed by addition of enzyme conjugate (T4 labelled with HRPO).

Binding of T4 is detected by Enzyme Conjugate. Incubation is followed by a washing step to remove unbound components. The color reaction is started by addition of substrate and stopped after a defined time. The color intensity is inversely proportional to the concentration of T4 in the sample.

Ordering Information

Performance Characteristics (Internal Evaluation) Of T4 Quanti Microlisa Test

1. Accuracy : The kit has been tested using clinical samples in comparison with commercially available reference immunoassay test kits (ELISA /CLIA/ELFA) and the Accuracy co-efficient is > 0.985
2. Precision :
   1. The test results are repeatable in Within run precision testing with CV <10%.
   2. The test results are reproducible in between run precision testing with CV <15%.