Test Kit Type:Total T3 Test Kit (CLIA)
Analytical Sensitivity:0.195 ng/ml
Linearity0.195 ng/ml to 7.5 ng/ml

Intended Use

T3 iClia is a chemiluminescent microparticle immunoassay designed for in vitro quantitative measurement of Total Triiodothyronine (T3) in human serum or plasma. This kit is only operational in conjunction with J. Mitra  CLIA Analyzer.

Salient Features

  • Based on Competitive chemiluminescent microparticle immunoassay
  • Acridinium Ester (AE) based kit for more accurate results
  • Test result within 30 minutes
  • Analytical Sensitivity: 195 ng/ml
  • Linearity: 195 ng/ml to 7.5 ng/ml
  • Long shelf life: 12 months at 2-8oC
  • Onboard stability of reagents: 30 days at 2-8oC
  • Convenient pack size: 50 Tests & 100 Tests
  • Prompt service back-up


T3 iClia is chemiluminescent immunoassay based on the “competitive” principle. The magnetic microspheres are coated with Anti-triiodothyronine (T3) antibodies.

The Assay Buffer and samples are mixed and added in the assay cup containing anti-T3 antibodies coated microspheres. Next, T3 conjugated acridinium ester is added. T3 of labelled AE and T3 present in sample compete for binding sites of anti-T3 antibodies coated magnetic microspheres. After further incubation and washing, Pre-Trigger and Trigger Solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative Light units (RLUs). There is an indirect relationship between the amount of T3 present in the sample and the RLUs detected by the optical system. Results are calculated automatically based on the established calibration curve.


Performance Characteristics

Limit of Blank (LoB):  ≤0.195 ng/ml

Accuracy: The accuracy of T3 iClia is tested with 60 clinical specimens and with Roche CLIA. The accuracy co-efficient is ≥0.970.


  1. The test results are repeatable in within run precision testing with CV <6%.
  2. The test results are reproducible in between run precision testing with CV <10%.

Ordering Information

Product Name Pack Size Catalog No.
T3 iClia 50 Tests IR021050
T3 iClia 100 Tests IR021100

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