Test Kit Type: ELISA Test Kit
Sensitivity: 100%
Specificity: 100%
Assay Procedure: 90 Minutes

Intended Use

Syphi Microlisa is an in-vitro qualitative enzyme immunoassay for the detection of antibodies to Treponema pallidum (TP) in human serum or plasma. It is intended for screening of blood donors and for clinical diagnostic testing.

  • Salient Features
  • Based on Sandwich Elisa principle.
  • Colour coded reagents to monitor procedural steps.
  • Ready to Use Enzyme Conjugate.
  • Total test time: 90 minutes.
  • Longer Shelf life: 18 months at 2-8°C


Syphi Microlisa test is an enzyme immunoassay based on sandwich ELISA. Syphilis antigen are coated onto microtitre wells. Specimens and controls are added to the microtitre wells and incubated. Antibodies to syphilis if present in the specimen will binds the specific antigens absorbed onto the surface of the wells. The plate is then washed to remove unbound material. Horseradish peroxidase (HRP) conjugated syphilis antigen added to each well. This conjugate will bind syphilis antigen-antibody complex present. Finally substrate solution containing chromogen and hydrogen peroxide is added to wells and incubated.

A blue colour will develop in proportion to the syphilis antibodies present in the specimen. The colour reaction is stopped by a stop solution. The enzyme substrate reaction is read by EIA reader for absorbance at a wavelength of 450/630nm . If the sample does not contain syphilis antibodies then enzyme conjugate will not bind and the solution in the wells will be either colorless or only a faint background colour develops.

Ordering Information

Product Name Pack Size Catalog No.
Syphi Microlisa 96 Tests IR012096


Performance of the test with reference to sensitivity and specificity has also been  evaluated in-house with the panel of 286 negative samples, 50 Syphilis Positive and 10 potentially cross-reacting specimen; HIV,HCV, Dengue, Chikungunya, Leptospira positive samples .The results obtained are as follows:

Sensitivity: 100%

Specificity: 100%

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