PCT Quanti Microlisa

Intended Use

PCT Quanti Microlisa is designed for in-vitro quantitative determination of PCT in human serum or plasma.

Salient Features

• Based on sandwich Immunoassay.
• Quantitative Detection of PCT(Procalcitonin) in Human Serum/Plasma.
• Linearity Range: 0.010- 25 ng/ml.
• Analytical sensitivity is 0.010 ng/ml.
• Breakaway microwell strips.
• Color coded reagents to monitor procedural steps.
• Assay procedure : 45min.
• Prompt service back-up.
• Longer shelf life : 18 month at 2-8°C.
• Pack size: 96 test.

Principle

PCT Quanti Microlisa is an enzyme immuno assay based on sandwich ELISA.Microwells are pre-coated with antibodies against human procalcitonin.

Sample is added to the microwell followed by addition of enzyme conjugate (anti-PCT antibodies labelled with HRPO). Binding of PCT is detected by enzyme conjugate. Incubation is followed by a washing step to remove unbound components. The color reaction is started by addition of substrate and stopped after a defined time. The color intensity is directly proportional to the concentration of PCT in the sample.

Ordering Information

Performance Characteristics Of PCT Quanti Microlisa Test

1. Accuracy : The kit has been tested using clinical samples in comparison with commercially available reference immunoassay test kits (ELISA /CLIA/ELFA) and the Accuracy co-efficient is > 0.996
2. Precision :
   1. The test results are repeatable in Within run precision testing with CV <10%.
   2. The test results are reproducible in between run precision testing with CV <15%.