Test Kit Type: Malaria ELISA Test Kit
Shelf Life: 24 Months at 2-8°C
Feature: Color coded reagents
Assay Procedure: 120 Minutes

Intended Use

Malaria Ag Microlisa is an in-vitro qualitative enzyme immunoassay for detecting infection with plasmodium species (P.falciparum /P.vivax/ P.malariae/ P.ovale) based on pLDH antigen in human blood. It is intended for screening of blood donors or other individuals at risk for malaria infection and for clinical diagnostic testing.

Salient Features

  • Detection of plasmodium species (P.falciparum /P.vivax/ P.malariae/ P.ovale) based on pLDH antigen in human blood
  • Break away wells for minimal wastage
  • Easy interpretation of results
  • System configuration compatible with all common ELISA equipment
  • High analytical Sensitivity of >20 parasites/ul of blood
  • Shelf life: 24 Months at 2-8°C
  • Color coded reagents to monitor procedural steps
  • Assay procedure: 120mins


Malaria Ag Microlisa test is an immunoassay based on sandwich ELISA. Anti-Pan (pLDH) antibodies has been coated onto the wells of the microwell plate provided. Blood sample including controls are pipetted into these wells. During the first incubation, the plasmodium species pLDH antigen from samples and coated antibody react and after washing the enzyme conjugate (anti-pLDH antibody HRPO) is added. After incubation washing is done to remove all the unbounded enzyme conjugate.

A substrate solution which acts on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of pLDH antigen present in the samples.

Ordering Information

Product Name Pack Size Catalog No.
Malaria Ag Microlisa 96 Tests IR370096

Performance Characteristics (In House Evaluation)

The Malaria Ag Microlisa test kit has been evaluated in-house with malaria positive and negative clinical whole blood samples and compared with microscopic examination. The evaluation also included cross-reacting samples; Dengue, Rheumatoid factor, Leptospira, HIV, HCV, HBV, M. tuberculosis, Syphilis, Brucella, Scrub typhus positive samples. The results obtained are as follows:

Samples No. of Samples Malaria Ag Microlisa Sensitivity (%) Specificity (%)
+ve -ve
Malaria Negative 100 0 100 100
P. falciparum Positive 10 10 0 100
P. vivax Positive 30 30 0 100

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