Intended Use
IL-6 iClia Diagnostic Kit (AE) is intended for the in vitro quantitative measurement of Interleukin-6 (IL-6) in human serum/plasma as an aid in the monitor of the immune state and inflammatory response of the body in conjunction with other laboratory and clinical findings. This kit is only operational in conjunction with J. Mitra CLIA Analyzer.
Salient Features
- Based on Sandwich chemiluminescent microparticle immunoassay
- Acridinium Ester (AE) based kit for more accurate results
- Test result at 25 minutes
- Analytical sensitivity : 2.00 pg/mL
- Linearity: 2.00 pg/mL – 5000.00 pg/mL
- Long shelf life: 12 months at 2-8oC
- Onboard stability of reagents: 30 days at 2-8oC
- Convenient pack size: 50 Test Pack
- Prompt service back-up
Principle
IL-6 iClia kit is a chemiluminescent based on “Sandwich principle”. The magnetic microparticles are coated with ant IL-6 antibodies. The samples are added in the assay cup containing assay buffer and anti-IL6 antibodies coated microspheres followed by addition of AE conjugate (anti-IL-6 labeled acridinium ester conjugate) to assay cup. A sandwich complex is formed wherein IL-6(from serum/plasma sample) is “trapped” or “sandwiched” between the microspheres coated antibody and antibody labelled with AE conjugate. Unbound conjugate is then washed off with the wash buffer. The amount of bound AE conjugate is proportional to the concentration of IL-6 present in the sample. Finally pre-trigger and trigger solution containing hydrogen peroxide and sodium hydroxide solution is added to reaction mixture. The resulting
Chemiluminescent reaction is measured as relative light units (RLUs). There is a direct relationship between the amount of IL-6 present in the sample and RLUs detected by the optical system. Results are calculated automatically based on the established calibration curve.