HIV Ag&Ab CARD (4TH Generation)

Test Kit Type: HIV Antigen and Antibody Rapid Test Kit
Feature: 4th Generation , Use of capillary and venous samples
Detect HIV -1 p24 Antigen and Antibodies (IgM, IgG& IgA) Detection

Intended Use

HIV Ag & Ab Card (4th Generation) is a visual, rapid qualitative and sensitive solid phase
immunochromatographic assay for the differential detection of HIV-1 p24 Antigen and Antibodies (IgM, IgG &
IgA) to HIV-1& HIV-2 in Human Serum/ Plasma/ Whole blood (collected by venipuncture or Fingerprick). The
test is a screening test for p24 antigen (HIV-1) and HIV antibodies (anti-HIV-1 & anti-HIV-2) and is for in vitro
diagnostic use only. It is intended for screening of blood donors or other individuals at risk for HIV-1 & HIV-2
infection and for clinical diagnostic testing. It is a screening test for p24 antigen (HIV-1) and HIV antibodies
(anti-HIV-1 & anti-HIV-2). The test is for in vitro diagnostic use only and is intended for screening of blood
donors or others individuals at risk for HIV-1 and/or HIV-2 infection and for clinical diagnostic testing.

Salient Features

• Finger prick, rapid, visual and qualitative Immunoassay.
• 4TH Generation Rapid test for differential detection of p24 Antigen & antibodies for HIV in serum/plasma /whole blood.
• Ideal for Blood screening, detects sero-negative HIV Cases.
• Use of gp-41, C terminus of gp120 for HIV-1 , gp-36 for HIV -2 antibodies and Anti-p24 for HIV p24 Ag detection.
• Detection of Group ‘0’ & subtype ‘C’ of HIV-1, which is most prevalent in India.
• Based on Immunochromatography Technique.
• See through device for easy interpretation of results.
• Sensitivity : 100 % & Specificity: 100%
• Analytical Sensitivity of HIV p24 Ag : 100 IU/ml (traceable to p24 Antigen NIBSC Standard, U.K. Code No. 90/636)
• Long Shelf life 30 months at 2-30°C
• Convenient pack size: 25 T.

Principle

HIV Ag & Ab (4th Generation) Test kit consists two devices; one device for detection of HIV-1 p24 antigen and
second device for the detection of HIV-1 & HIV-2 antibodies in human serum/ plasma/whole blood.
HIV Antigen device contains two lines; “C” (Control Line) & “Ag” (HIV-1 p24 Antigen detection Test Line). Test
line is coated with anti-p24 antibodies. When a sample is added to the HIV Antigen device followed by the
addition of assay buffer, HIV-1 p24 antigen if present in the sample will bind to the anti-p24 gold colloid
conjugate making antigen antibodies complex. This complex migrates along the membrane to the test region
and forms the visible purple line at “Ag” region as antibody-antigen-antibody gold conjugate complex.
HIV Antibody test device contains two lines; “C” (Control line), & “Ab” (HIV-1 & HIV-2 Antibody detection Test
Line). The test line is coated with HIV-1 & HIV-2 Antigens for the detection of HIV Antibodies. When a sample is
added to the HIV Antibody device followed by the addition of assay buffer, anti-HIV-1 and/or anti-HIV-2
antibodies if present in the sample, bind to the respective HIV-1 and/or HIV-2 antigen gold colloid conjugate
making antigen-antibodies complexes. These complexes migrate along the membrane to the test region and
form the visible purple line at “Ab” region as antigen-antibody-antigen gold colloid conjugate complex.
The intensity of the test bands in the respective device will vary depending upon the amount of antigen/antibody present in the sample.
The appearance of any pink/purple color line in a specific test region should be considered as reactive for that particular antigen and/or antibody. A red procedural control line
should always develop in the test device window to indicate that the test has been performed properly.

Ordering Information

Product Name Pack Size Catalog No.
HIV Ag&Ab CARD (4TH Generation) 25 Tests IR038025
50 Tests IR038050

 

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