Products Details

• Detection of plasmodium species (P.falciparum /P.vivax/ P.malariae/ P.ovale) based on pLDH antigen in human blood
• Break away wells for minimal wastage
• Easy interpretation of results
• System configuration compatible with all common ELISA equipment
• High analytical Sensitivity of >20 parasites/ul of blood
• Shelf life: 18 months at 2-8°C
• Color coded reagents to monitor procedural steps
• Assay procedure: 120mins

Malaria Ag Microlisa test is an immunoassay based on sandwich ELISA.
Anti-Pan (pLDH) antibodies has been coated onto the wells of the microwell plate provided. Blood sample including controls are pipetted into these wells. During the first incubation, the plasmodium species pLDH antigen from samples and coated antibody react and after washing the enzyme conjugate (anti-pLDH antibody HRPO) is added .After incubation washing is done to remove all the unbounded enzyme conjugate. A substrate solution which acts on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of pLDH antigen present in the samples.

ORDERING INFORMATION

PERFORMANCE CHARACTERISTICS 

In-house evaluation

The Malaria Ag Microlisa test kit has been evaluated in-house with malaria positive and negative clinical whole blood samples and compared with microscopic examination. The evaluation also included cross-reacting samples; Dengue, Rheumatoid factor, Leptospira, HIV, HCV, HBV, M. tuberculosis, Syphilis, Brucella, Scrub typhus positive samples. The results obtained are as follows: